ANTI-INTEGRIN BETA1 ANTIBODY; CLONE 12G1

Analysis Note

ControlHeLa cells

Application

Research CategoryCell Structure

This Anti-Integrin β1 Antibody, clone 12G10 is validated for use in Western Blotting, Flow Cytometry, Immunofluorescence for the detection of Integrin β1.

Research Sub CategoryECM Proteins

Western Blot Analysis: 0.5 µg/mL from a representative lot detected Integrin β1 in 10 µg of Integrin purified protein.

Immunofluorescence Analysis: A representative lot was used in an independent laboratory in mouse lung tissue. (Chen P., et al. (2009). PLoS ONE. 4(8):e6565.)

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Integrin beta-1 (UniProt P05556; also known as CD29, Glycoprotein Iia, GPIIA, Integrin VLA-4 subunit beta, Very late activation protein, beta polypeptide) is encoded by the ITGB1 (also known as FNRB, MDF2, MSK12, VLA-BETA, VLAB) gene (Gene ID 3688) in human. Integrin beta-1 is a 130 kDa transmembrane glycoprotein that interact with various integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6) to form the functional receptor complexes that bind to specific extracellular matrix proteins. Integrin receptors regulate a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor growth and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. Interactions between integrins and the extracellular matrix lead to the activation of signal transduction pathways and regulation of gene expression.

Immunogen

Purified human β1 Integrin preparation from HT1080 fibrosarcoma cell extract

Linkage

Replaces: MAB2247

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified mouse monoclonal IgG1κ in PBS containing 0.1% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Flow Cytometry in HeLa cells.

Flow Cytometry Analysis: 1 µg of this antibody detected Integrin β1 in HeLa cells.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~192 kDa observed